Characterization of the human gene encoding α-aminoadipate aminotransferase (AADAT)

摘要: Abstract In mammals, the conversion of α-aminoadipate to α-ketoadipate by aminotransferase (AADAT) is an intermediate step in lysine degradation. A gene encoding for and kynurenine activites had been previously identified rat ( KAT/AadAT ). We human AADAT ) AADAT. It has a 2329 bp cDNA, 1278 bp open-reading frame, predicted encode 425 amino acids with mitochondrial cleavage signal pyridoxal-phosphate binding site. 73% 72% identical mouse orthologs, respectively. The genomic structure spans 30 kb consists 13 exons. FISH studies localized 4q32.2. Two transcripts (∼2.9 ∼4.7 kb were identified, expression highest liver. Bacterial confirm that encodes activity. availability DNA sequence enzyme assay will allow further evaluation individuals suspected have defects this enzyme.