Differential Sensitivity of Glycogenolysis and Gluconeogenesis to Insulin Infusions in Dogs

摘要: The suppressive effect of insulin on hepatic glucose production is generally recognized. Though it well established that this at least partially due to inhibition glycogenolysis, controversy still exists about insulin9s gluconeogenesis. present study was undertaken determine whether could affect gluconeogenesis from alanine in the intact dog and compare glycogenolysis In anesthetized dogs fasted overnight, blood samples were drawn simultaneously a femoral artery vein. Alanine-U- 14 C, 10 μCi./kg., infused over 110 minutes. A constant infusion either 1 or 5 mU./kg./min. begun 50 minutes, concentration maintained by variable infusion. When mU./kg./min., resulting plasma immunoreactive (IRI) levels 73 ± μU./ml., net splanchnic (NSGP) suppressed 2.7 2 mg./kg./min. virtually zero. constrast, small increment had no demonstrable uptake conversion (7.9 0.3 μmol/min.). Insulin resulted IRI 240 25 muU./ml. This higher associated with marked suppression both NSGP (100 per cent) (90 but did not extraction bed. Doses 35 cent fall glucagon levels. These data demonstrate (1) more sensitive than inhibitory increments concentrations, (2) be only (3) an intrahepatic rather alanine, finally, (4) can suppress absence hyperglycemia.