Detection of Effects Caused by Very Low Levels of Contaminants in Riverine Sediments Through a Combination of Chemical Analysis, In Vitro Bioassays, and Farmed Fish as Sentinel

摘要: Aquatic organisms are often exposed to mixtures of low levels pollutants whose presence and effects can pass easily unnoticed if only traditional monitoring strategies employed. The main aim this work was assess the trace in a scarcely affected area through combination chemical biological approaches. Sediments were collected along river with little anthropogenic pressure assayed for cytochrome P450 (Cyp1a)-dependent ethoxyresorufin-O-deethylase (EROD) activity rainbow trout gonadal cell line RTG-2. Chemical analyses performed these sediments using two-dimensional gas chromatography-time-of-flight mass spectrometry. Sediment samples induced EROD activity, evidenced wide variety contaminants range nanograms per gram dry weight. Correlation analysis between induction data showed an r value 0.840 (p < 0.05). In addition, fish from farm located downstream sampling points exhibited high hepatic as well expression cyp1a cyp3a. conclusion, appropriate techniques allowed detection theoretically nonaffected river.