Cox 2 community barcoding at Prince Edward Island reveals long-distance dispersal of a downy mildew species and potentially marine members of the Saprolegniaceae

摘要: Marine oomycetes are highly diverse, globally distributed, and play key roles in marine food webs as decomposers, source, parasites. Despite their potential importance global ocean ecosystems, comparatively little studied. Here, we tested if the primer pair cox2F_Hud cox2-RC4, which is already well-established for phylogenetic investigations of terrestrial oomycetes, can also be used high-throughput community barcoding. Community barcoding a plankton sample from Brudenell River (Prince Edward Island, Canada), revealed six distinct oomycete OTU clusters. Two these clusters corresponded to members Peronosporaceae—one could assigned Peronospora verna, an obligate biotrophic pathogen plant Veronica serpyllifolia related species, other was closely Globisporangium rostratum. While detection former likely due long-distance dispersal island, latter might bona fide several cultivable species Peronosporaceae known withstand high salt concentrations. lineages Saprolegniaceae. represent otherwise genus, it conceivable that they were introduced on detritus island. additional grouped with early-diverging but not specific family. This reflects current underrepresentation cox2 sequence data will hopefully improve increasing interest oomycetes.