Optimizing of Synthetic Seed of Malling apple (Malusdomestica) Rootstocks cv. M26 and cv. MM106

摘要: Malling Increasing demands for apple across the world requires its rapid production. Agricultural biotechnology has met this need and prevented some problems during adolescence period long generation. We investigated embryonic non-embryonic callus through cultivating apical lateral buds on different treatments with MS base culture BAP (0-2.5 mg/l) IBA (0-5 hormones Kin NAA hormones. The highest percent of embryogenesis related to T11 treatment (IBA 4 mg/l, 1 T23 2.5 mg/l). embryos were in corpuscular stage. Then third sub-culture was performed all calluses delivered their corresponding treatments. K5 (NAA 0.5 K8 3 yielded amount (5.3 mm). remained stage they did not show reproductive ability developmental process. After preparation artificial seed no regeneration seen. By MM.106 M.26, purpose research is improving a protocol generation two somatic cultivars.