Environmental genomics of C1 metabolism.

摘要: Facultative methylotrophs are capable of growth on multicarbon compounds, while obligate not. Knowledge the content and structure genomes methylotrophic bacteria is instrumental for detecting C1 metabolism genes in environment. Comparative analysis methylotroph genomes, as well proteomic analyses, provides knowledge complement essential The current methylotrophy modules, however, remains incomplete. While originally developed detection copper-containing membrane-bound methane monooxygenase (pMMO), this protocol may be adapted to detect other encoding key enzymes metabolism. Detection specific groups based light scattering and/or autofluorescence. In absence natural autofluorescence, microbial cells can differentiated by various types fluorescence staining, using either immunofluorescent labeling or fluorescent situ hybridization (FISH). first prohibitive cost, second computational difficulty assembling large databases random DNA sequences . progress environmental genomics will bring about new clues possible means cultivating microbes that have so far resisted cultivation. field still its infancy, it easy predict future full surprises.