Biosynthesis of proinsulin in islets of Langerhans of the carp (Cyprinus carpio).
作者： Ansgar Lukowsky , Siegfried Prehn , Tom A. Rapoport
DOI: 10.1016/0005-2795(74)90221-9
关键词:
摘要: Abstract Islets of Langerhans from carp were incubated with [U- 14 C]leucine at 15 °C for 2 h. About 3–4% the newly synthesized protein was proinsulin. The identity proved by: (1) its high specific radioactivity, (2) a molecular weight 10 000, (3) co-chromatography porcine insulin after tryptic digestion, (4) cleavage digest into two chains which co-chromatograph A- and B-chains (5) by failure original, non-trypsinated chains. A conversion proinsulin not observed.
elsevier.com
sci-hub.se 参考文章(6)
P T Grant, K. B. M. Reid, Biosynthesis of an insulin precursor by islet tissue of cod (Gadus callarias) Biochemical Journal. ,vol. 110, pp. 281- 288 ,(1968) , 10.1042/BJ1100281
Kenji Yamaji, Kozue Tada, Anthony C. Trakatellis, On the Biosynthesis of Insulin in Anglerfish Islets Journal of Biological Chemistry. ,vol. 247, pp. 4080- 4088 ,(1972) , 10.1016/S0021-9258(19)45141-7
Rene E. Humbel, Studies on isolated islets of Langerhans (Brookmann bodies) of teleost fishes II. Evidence for insulin biosynthesis in vitro Biochimica et Biophysica Acta. ,vol. 74, pp. 96- 104 ,(1963) , 10.1016/0006-3002(63)91333-7
Margaret L. Moule, Cecil C. Yip, Insulin biosynthesis in the bullhead, Ictalurus nebulosus, and the effect of temperature Biochemical Journal. ,vol. 134, pp. 753- 761 ,(1973) , 10.1042/BJ1340753
K. B. M. Reid, P T Grant, A. Youngson, The sequence of amino acids in insulin isolated from islet tissue of the cod (Gadus callarias) Biochemical Journal. ,vol. 110, pp. 289- 296 ,(1968) , 10.1042/BJ1100289
Coombs Tl, Grant Pt, Proinsulin, a biosynthetic precursor of insulin. Essays in Biochemistry. ,vol. 6, pp. 69- 92 ,(1970)