High frequency stimulation abolishes thalamic network oscillations: an electrophysiological and computational analysis

摘要: Deep Brain Stimulation (DBS) of thalamus has been demonstrated to be an effective for treatment epilepsy. To investigate mechanism action thalamic DBS, we examined the effects high frequency stimulation (HFS) on spindle oscillations in brain slices from ferrets. We recorded intracellular and extracellular electrophysiological activity nucleus Reticularis thalami (nRt) thalamocortical relay (TC) neurons lateral geniculate nucleus, stimulated slice using a concentric bipolar electrode, level glutamate within slice. HFS (100 Hz) TC generated excitatory post-synaptic potentials (EPSPs), increased number both nRt neurons, reduced input resistance, concentration, abolished wave oscillations. High also suppressed In locations, was associated with significant persistent elevation levels many seconds after cessation stimulation. simulated computational model network, disrupted activity, but suppression short-lived. Simulated prolonged periods time only release glutamate-mediated activation hyperpolarization-activated current (Ih) were incorporated into model. Our results suggest that DBS as used epilepsy may involve glutamate, which turn modulates specific ion channels such Ih, decreases neuronal abolishes network oscillatory activity.