Characterization of the Upper Respiratory Tract Microbiomes of Patients with Pandemic H1N1 Influenza

摘要: The upper respiratory tract microbiome has an important role in health. Influenza A is a common viral infection that challenges health, and well-recognized sequela bacterial pneumonia. Given this connection, we sought to characterize the microbiota of individuals suffering from pandemic H1N1 influenza outbreak 2009 determine if profiles could be correlated with patient characteristics. We determined microbial 65 samples patients by cpn60 universal target amplification sequencing. Profiles were examined at phylum nearest neighbor “species” levels using characteristics gender, age, originating health authority, sample type designation (STAT/non-STAT). At level, Actinobacteria-, Firmicutes- Proteobacteria-dominated microbiomes observed, none showing significant profile composition differences. composed 13-20 showed trend towards increasing diversity age. Interestingly, individual most had one three organisms dominant their microbiota. limited number discrete shared among regardless status variables. To assess validity analyses derived sequence read abundance, several species quantified quantitative PCR compared abundance counts obtained study. strong positive correlation between absolute quantification was observed. This study represents first examination other than 16S rRNA gene our knowledge, thorough during infection.