Plasma membrane assays and three-compartment image cytometry for high content screening.
作者： Natalie L. Prigozhina , Ling Zhong , Edward A. Hunter , Ivana Mikić , Scott Callaway
DOI: 10.1089/ADT.2006.024
关键词:
摘要: Abstract: High throughput image cytometers analyze individual cells in digital photomicrographs by first assigning pixels within each to plasma membrane, cytoplasm, nucleus, or other regions. In this study, we report on a novel algorithm that: 1) identifies membrane regions measure changes membrane-associated proteins (protein kinase C [PKC] α, N-cadherin, E-cadherin, vascular endothelium [VE]-cadherin, and pan-cadherin) that regulate cell division, migration, adhesion 2) delineates the for generalized three-compartment cytometry. Validation assays were performed these cultured 96-well plates also tissue sections obtained from transgenic chemical carcinogenic models of skin cancer. The successfully quantified phorbol 12-myristate 13-acetate (PMA)-induced localization PKCα HeLa (Z′ 0.88). Additionally, PMA activated translocation at P < .01 N-cadherin (in HeLa...
liebertonline.com参考文章(60)
Ugo Cavallaro, N-cadherin as an invasion promoter: a novel target for antitumor therapy? Current opinion in investigational drugs. ,vol. 5, pp. 1274- 1278 ,(2004)
D R Roop, C K Cheng, L Titterington, C A Meyers, J R Stanley, P M Steinert, S H Yuspa, Synthetic peptides corresponding to keratin subunits elicit highly specific antibodies. Journal of Biological Chemistry. ,vol. 259, pp. 8037- 8040 ,(1984) , 10.1016/S0021-9258(17)39682-5
Patrick M. Mcdonough, Ivana Mikic, Jeffrey H. Price, Edward Hunter, System, method and kit for processing a magnified image of biological material to identify components of a biological object ,(2006)
Ryoko Okamoto, Kenji Irie, Akio Yamada, Tatsuo Katata, Atsunori Fukuhara, Yoshimi Takai, Recruitment of E-cadherin associated with alpha- and beta-catenins and p120ctn to the nectin-based cell-cell adhesion sites by the action of 12-O-tetradecanoylphorbol-13-acetate in MDCK cells. Genes to Cells. ,vol. 10, pp. 435- 445 ,(2005) , 10.1111/J.1365-2443.2005.00846.X
Ivana Mikic, Sonia Planey, Jun Zhang, Carolina Ceballos, Terri Seron, Benedikt von Massenbach, Rachael Watson, Scott Callaway, Patrick M. McDonough, Jeffrey H. Price, Edward Hunter, David Zacharias, A live cell, image-based approach to understanding the enzymology and pharmacology of 2-bromopalmitate and palmitoylation. Methods in Enzymology. ,vol. 414, pp. 150- 187 ,(2006) , 10.1016/S0076-6879(06)14010-0
T. Volberg, I. Sabanay, R.O. Hynes, D. Ginsberg, B. Geiger, S. Bitzur, Broad spectrum pan-cadherin antibodies, reactive with the C-terminal 24 amino acid residues of N-cadherin Journal of Cell Science. ,vol. 97, pp. 607- 614 ,(1990)
Janice L. Walker, Alaina K. Fournier, Richard K. Assoian, Regulation of growth factor signaling and cell cycle progression by cell adhesion and adhesion-dependent changes in cellular tension Cytokine & Growth Factor Reviews. ,vol. 16, pp. 395- 405 ,(2005) , 10.1016/J.CYTOGFR.2005.03.003
DaoHai Zhang, Manuel Salto-Tellez, Thomas Choudary Putti, Elaine Do, Evelyn Siew-Chuan Koay, Reliability of Tissue Microarrays in Detecting Protein Expression and Gene Amplification in Breast Cancer Modern Pathology. ,vol. 16, pp. 79- 85 ,(2003) , 10.1097/01.MP.0000047307.96344.93
Karel OTERO, Fernando MARTÍNEZ, Amada BELTRÁN, Deyarina GONZÁLEZ, Beatriz HERRERA, Gypsy QUINTERO, René DELGADO, Armando ROJAS, Albumin-derived advanced glycation end-products trigger the disruption of the vascular endothelial cadherin complex in cultured human and murine endothelial cells Biochemical Journal. ,vol. 359, pp. 567- 574 ,(2001) , 10.1042/0264-6021:3590567
Fiemu E Nwariaku, Jianping Chang, Xudong Zhu, Zijuan Liu, Steven L Duffy, Nabil H Halaihel, Lance Terada, Richard H Turnage, None, The role of p38 map kinase in tumor necrosis factor-induced redistribution of vascular endothelial cadherin and increased endothelial permeability. Shock. ,vol. 18, pp. 82- 85 ,(2002) , 10.1097/00024382-200207000-00015