Rift Valley fever virus MP-12 vaccine encoding Toscana virus NSs retains neuroinvasiveness in mice.
作者： Sabarish V. Indran , Olga A. Lihoradova , Inaia Phoenix , Nandadeva Lokugamage , Birte Kalveram
关键词:
摘要: Rift Valley fever is a mosquito-borne zoonotic disease endemic to sub-Saharan Africa. virus (RVFV; genus Phlebovirus, family Bunyaviridae) causes high rates of abortion and fetal malformation in pregnant ruminants, haemorrhagic fever, neurological disorders or blindness humans. The MP-12 strain highly efficacious safe live-attenuated vaccine candidate for both humans ruminants. However, lacks marker differentiate infected from vaccinated animals. In this study, we originally aimed characterize the efficacy recombinant RVFV encoding Toscana (TOSV) NSs gene place (rMP12-TOSNSs). TOSV promotes degradation dsRNA-dependent protein kinase (PKR) inhibits interferon-β up-regulation without suppressing host general transcription. Unexpectedly, rMP12-TOSNSs increased death outbred mice inbred BALB/c C57BL/6 mice. Immunohistochemistry showed diffusely positive viral antigens thalamus, hypothalamus brainstem, including medulla. No were detected spleen liver, which similar antigen distribution moribund with MP-12. These results suggest that retains neuroinvasiveness Our findings demonstrate neuroinvasion any hepatic will be useful studying mechanism TOSV.
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