The introduction of a negative charge into the hydrophobic patch of Pseudomonas aeruginosa azurin affects the electron self-exchange rate and the electrochemistry.

摘要: By changing Met64 into a glutamate by means of site-directed mutagenesis negative charge was introduced the hydrophobic patch azurin from Pseudomonas aeruginosa. The three-dimensional structure protein and metal site in particular, appear unaffected mutation. observed change midpoint potential mutant 28 mV is ascribed to deprotonation Glu64. electron-self-exchange rate constant equals that wild-type at pH 4.5 but decreases almost two orders magnitude high pH. Electron transfer inhibited only when both reacting molecules have an ionized position 64 their patch. graphite electrode slowed down presence results demonstrate once again Cu-ligand His117 likely entry exit point for electrons. This observation holds (homogeneous) reaction solution as well (heterogenous) electrode.