Metabolomic response of human skin tissue to low dose ionizing radiation
作者： Ze-Ping Hu , Young-Mo Kim , Marianne B. Sowa , R. Joe Robinson , Xiaoli Gao
DOI: 10.1039/C2MB25061F
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摘要: Understanding how human organs respond to ionizing radiation (IR) at a systems biology level and identifying biomarkers for IR exposure low doses can help provide scientific basis establishing protection standards. Little is known regarding the physiological responses dose metabolite level, which represents end-point of biochemical processes inside cells. Using full thickness skin tissue model GC-MS-based metabolomic analysis, we examined metabolic perturbations three time points (3, 24 48 h) after 3, 10 200 cGy X-rays. PLS-DA score plots revealed dose- time-dependent clustering between sham irradiated groups. Importantly, delayed were observed IR. When compared with high cGy, comparable number significantly changed metabolites detected h (3 cGy) irradiation. Biochemical pathway analysis showed DNA/RNA damage repair, lipid energy metabolisms, even
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Yanrong Su, Jarah A. Meador, Charles R. Geard, Adayabalam S. Balajee, Analysis of ionizing radiation-induced DNA damage and repair in three-dimensional human skin model system Experimental Dermatology. ,vol. 19, ,(2009) , 10.1111/J.1600-0625.2009.00945.X
Esther Boelsma, Susan Gibbs, Maria Ponec, Claudine Faller, Characterization and Comparison of Reconstructed Skin Models: Morphological and Immunohistochemical Evaluation Acta Dermato-venereologica. ,vol. 80, pp. 82- 88 ,(2000)
Yu Cheng, Guoxiang Xie, Tianlu Chen, Yunping Qiu, Xia Zou, Minhua Zheng, Binbin Tan, Bo Feng, Taotao Dong, Pingang He, Linjing Zhao, Aihua Zhao, Lisa X. Xu, Yan Zhang, Wei Jia, Distinct urinary metabolic profile of human colorectal cancer. Journal of Proteome Research. ,vol. 11, pp. 1354- 1363 ,(2012) , 10.1021/PR201001A
Timothy M. D. Ebbels, Hector C. Keun, Olaf P. Beckonert, Mary E. Bollard, John C. Lindon, Elaine Holmes, Jeremy K. Nicholson, Prediction and Classification of Drug Toxicity Using Probabilistic Modeling of Temporal Metabolic Data: The Consortium on Metabonomic Toxicology Screening Approach Journal of Proteome Research. ,vol. 6, pp. 4407- 4422 ,(2007) , 10.1021/PR0703021
Leon Mullenders, Mike Atkinson, Herwig Paretzke, Laure Sabatier, Simon Bouffler, Assessing cancer risks of low-dose radiation Nature Reviews Cancer. ,vol. 9, pp. 596- 604 ,(2009) , 10.1038/NRC2677
Oscar Yanes, Julie Clark, Diana M Wong, Gary J Patti, Antonio Sánchez-Ruiz, H Paul Benton, Sunia A Trauger, Caroline Desponts, Sheng Ding, Gary Siuzdak, Metabolic oxidation regulates embryonic stem cell differentiation Nature Chemical Biology. ,vol. 6, pp. 411- 417 ,(2010) , 10.1038/NCHEMBIO.364
John C. Lindon, Elaine Holmes, Mary E. Bollard, Elizabeth G. Stanley, Jeremy K. Nicholson, Metabonomics technologies and their applications in physiological monitoring, drug safety assessment and disease diagnosis Biomarkers. ,vol. 9, pp. 1- 31 ,(2004) , 10.1080/13547500410001668379
Ignasi Barba, Rafael Fernandez-Montesinos, David Garcia-Dorado, David Pozo, Alzheimer's disease beyond the genomic era: nuclear magnetic resonance (NMR) spectroscopy-based metabolomics Journal of Cellular and Molecular Medicine. ,vol. 12, pp. 1477- 1485 ,(2008) , 10.1111/J.1582-4934.2008.00385.X
Rency S. Varghese, Amrita Cheema, Prabhdeep Cheema, Marc Bourbeau, Leepika Tuli, Bin Zhou, Mira Jung, Anatoly Dritschilo, Habtom W. Ressom, Analysis of LC−MS Data for Characterizing the Metabolic Changes in Response to Radiation Journal of Proteome Research. ,vol. 9, pp. 2786- 2793 ,(2010) , 10.1021/PR100185B
Karsten Hiller, Jasper Hangebrauk, Christian Jäger, Jana Spura, Kerstin Schreiber, Dietmar Schomburg, MetaboliteDetector: comprehensive analysis tool for targeted and nontargeted GC/MS based metabolome analysis Analytical Chemistry. ,vol. 81, pp. 3429- 3439 ,(2009) , 10.1021/AC802689C