GATA-type transcriptional factor Gat1 regulates nitrogen uptake and polymalic acid biosynthesis in polyextremotolerant fungus Aureobasidium pullulans.
作者： Xiaodan Song , Yongkang Wang , Pan Wang , Guihong Pu , Xiang Zou
关键词:
摘要: Polymalic acid (PMA) is a novel biopolymer produced by the polyextremotolerant fungus Aureobasidium pullulans. In this study, GATA-family transcriptional factor, Gat1, which regulates nitrogen uptake and PMA biosynthesis, was investigated. production increased to 11.2% in mutant overexpressing gat1 but decreased 49.1% of titre when knocked out from genome A. Comparative transcriptome analysis wild-type strains (∆gat1 OE::gat1) revealed that 23 common differentially expressed genes were related oxidative phosphorylation, ribosome biogenesis, metabolism. Under nitrogen-limited stress, regardless preferred (glutamine, Gln) or non-preferred (proline, Pro), 70% Gat1 cells located nucleus-cytoplasm, resulted an increase biosynthesis regulation. Quantitative RT-PCR glucosekinase (GLK) glycolytic pathway malate synthase (MLS) glyoxylate shunt may be cross-regulated concentration (Gln Therefore, glk overexpressed strain (OE::gat1), yield 12.6% 13.0% respectively. These findings indicate play important role dual regulation carbon metabolisms biosynthesis.
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