Lipid droplets: a new player in colorectal cancer stem cells unveiled by spectroscopic imaging.
作者： Luca Tirinato , Carlo Liberale , Simone Di Franco , Patrizio Candeloro , Antonina Benfante
DOI: 10.1002/STEM.1837
关键词:
摘要: The cancer stem cell (CSC) model is describing tumors as a hierarchical organized system and CSCs are suggested to be responsible for recurrence after therapy. identification of specific markers therefore paramount importance. Here, we show that high levels lipid droplets (LDs) distinctive mark in colorectal (CR) cancer. This increased content was clearly revealed by label-free Raman spectroscopy it directly correlates with well-accepted CR-CSC CD133 Wnt pathway activity. By xenotransplantation experiments, have finally demonstrated CR-CSCs overexpressing LDs retain most tumorigenic potential. A relevant conceptual advance this work the demonstration cellular organelle, LD, signature CSCs, addition molecular markers. further functional characterization could lead soon design new target therapies against CR-CSCs. Stem Cells 2015;33:35–44
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