Assessment of Perigenital Sensitivity and Prostatic Mast Cell Activation in a Mouse Model of Neonatal Maternal Separation.

摘要: Chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) has a lifetime prevalence of 14% and is the most common urological diagnosis for men under age 50, yet it least understood studied chronic disorder. A significant subset patients with report having experienced early life stress or abuse, which can markedly affect functioning regulation hypothalamic-pituitary-adrenal (HPA) axis. Mast cell activation, been shown to be increased in both urine expressed prostatic secretions CP/CPPS patients, partially regulated by downstream activation HPA Neonatal maternal separation (NMS) used over two decades study outcomes rodent models, including changes axis visceral sensitivity. Here we provide detailed protocol using NMS as preclinical model male C57BL/6 mice. We describe methodology performing NMS, assessing perigenital mechanical allodynia, histological evidence mast activation. also that psychological have long-lasting effects on urogenital system