Prime-Boost Immunization of Rabbits with HIV-1 gp120 Elicits Potent Neutralization Activity against a Primary Viral Isolate

摘要: Development of a vaccine for HIV-1 requires detailed understanding the neutralizing antibody responses that can be experimentally elicited to difficult-to-neutralize primary isolates. Rabbits were immunized with gp120 subunit JR-CSF envelope (Env) using DNA-prime protein-boost regimen. We analyzed five sera showed potent autologous activity (IC50s at ∼103 104 serum dilution) against pseudoviruses containing Env from isolate but not related JR-FL. Pseudoviruses created by exchanging each variable and constant domain JR-FL or mutations in putative N-glycosylation sites. The contained different activities dependent on C3 V5, V4, V4 regions located glycan-rich outer gp120. All enhanced toward an variant lacked glycosylation site V4. epitopes recognized are generally distinct those several well characterized mAbs (targeting conserved sites Env) other type-specific V1, V2, V3 regions). one specific glycans also important 2G12 neutralization this blocked binding Our findings show fine specificities achieve HIV-1, yet strong does result improved breadth.