Transcription factors Asg1p and Hal9p regulate pH homeostasis in Candida glabrata

摘要: Candida glabrata is an important microorganism used in commercial fermentation to produce pyruvate, but very little known about its mechanisms for surviving acid stress culture. In this study, it was shown that transcription factors Asg1p and Hal9p play essential roles C. the tolerance of stress, as deletion CgASG1 or CgHAL9 resulted inability survive acidic environment. Cgasg1 Cghal9 mutant strains are unable maintain pH homeostasis, evidenced by a decrease intracellular increase reactive oxygen species production, which results metabolic disorders. The showed acidification partly due diminished activity plasma membrane proton pump, CgPma1p. addition, transcriptome sequencing revealed displayed variety changes gene expression under conditions, including genes MAPK signaling pathway, cell wall organization, trehalose accumulation, RIM101 pathway. Lastly, quantitative reverse-transcribed PCR cellular localization CgAsg1p CgHal9p played independent response stress.