Antimicrobial activity of amphiphilic neamine derivatives: Understanding the mechanism of action on Gram-positive bacteria.

摘要: Abstract Amphiphilic aminoglycoside derivatives are potential new antimicrobial agents mostly developed to fight resistant bacteria. The mechanism of action the 3′,6-dinonyl neamine, one most promising derivative, has been investigated on Gram-negative bacteria, including P. aeruginosa. In this study, we have assessed its against Gram-positive S. aureus and B. subtilis. By conducting time killing experiments, bactericidal effect induced by neamine MSSA MRSA. measuring displacement BODIPY™-TR cadaverine bound lipoteichoic acids (LTA), showed that interacts with these bacterial surface components. We also highlighted ability enhance membrane depolarization induce permeability, using fluorescent probes, DiSC3C(5) propidium iodide, respectively. These effects observed for both MRSA as well electronic microscopy, imaged disruption integrity cell wall fluorescence demonstrated changes in localization lipids from enriched-septum region impairment formation septum. At a glance, interferes multiple targets suggesting low bacteria acquire resistance agent. turn, amphiphilic candidates development novel multitarget therapeutic antibiotics.