Evaluation of the protective effects of α-tocopherol and retinol against ochratoxin A cytotoxicity

摘要: Ochratoxin A (OTA), a mycotoxin frequently present in food and feedstuffs, produces wide range of toxic effects, including cell death via lipid peroxidation. In one human four animal lines we determined the half lethal concentration (LC50) OTA, its effect on reactive oxygen species (ROS) production, ability to induce cytochrome p450 activity. We also examined protective alpha-tocopherol all-trans-retinol most sensitive (i.e. bovine mammary epithelia, for which LC50 was 0.8 microg/ml (24 h), Madin Darby canine kidney, 4.3 (48 h)). Pre-incubation 3 h with either antioxidant significantly (P<0.05) ameliorated OTA-induced reduction viability decreased ROS production. These findings indicate that oxidative stress is an important factor OTA cytotoxicity. Supplementation molecules may counteract short-term toxicity this mycotoxin.