Clonal analysis of human breast cancer by means of the polymerase chain reaction.
作者： Hideo Inaji , Hiroki Koyama , Kazuyoshi Motomura , Shinzaburo Noguchi , Shingi Imaoka
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摘要: Clonality of human breast cancer was analyzed in small DNA samples prepared from cryostat sections, by means the polymerase chain reaction (PCR). The method used for clonal analysis based on restriction fragment length polymorphism X-chromosome-linked phosphoglycerokinase (PGK) gene and differential methylation PGK due to random inactivation one two X-chromosomes females. All 20 PCR-based were monoclonal origin adjacent normal tissues polyclonal. When widely separated sites cancers, every sample found be monoclonal, always exhibiting same X-chromosome each tumor. study sensitivity showed that can detect presence cells against a polyclonal background when cell population is 50% or more. These results demonstrate PCR offers good studying clonality sections tumors. This could applied distinguish between benign (polyclonal) malignant (monoclonal) lesions.
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