Physical, chemical, and biological characterization of pulsed laser deposited and plasma sputtered hydroxyapatite thin films on titanium alloy.

摘要: The physical, chemical, and biological properties of pulsed laser deposited (PLD) plasma sputtered (PS) hydroxyapatite (HA) coatings were compared. Human osteoblast-like cell responses to these in vitro assayed for proliferation phenotypic expression. PS formed smooth continuous thin films that followed the contours substrate surface. PLD consisted numerous spheroidal micro- macroparticles. crystallinity all was quantified by comparison with HA target used both processes. XRD FTIR results indicated unannealed at room temperature had X-ray spectra consistent an amorphous structure found dissolve after only a few hours saline solution. Annealing 400 degrees C increased (87-98%), which resulted improved stability activity. showed greater chemical than contained approximate 15% crystalline phase, increasing 65% postannealing. Cell alkaline phosphatase production significantly higher on specimens other coating treatments. There may be benefits engineering presence minor percentage microcrystalline phase or nanometer scale polycrystalline structure.