In vitro culture of sugarcane infected with maize streak virus (MSV)

作者: Eric Bonnel , Jean-Pierre Péros , Bernard Reynaud

DOI: 10.1007/BF00034424

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摘要: Young buds and leaf tissues of sugarcane cv. R 574 (a Saccharum hybrid), healthy or infected with Maize Streak Virus (MSV) were cultured in vitro. The success rate the cultures from material is lower than that material. symptoms disease displayed on most plantlets produced but only 6% regenerated calli. indirect double-antibody sandwich ELISA technique using a monoclonal antibody makes it possible to identify virus-infected plantlets. In vitro, infection results reduced growth shoots.