作者: Gareth Griffiths , Gareth Griffiths
DOI: 10.1007/978-3-642-77095-1_4
关键词:
摘要: In those techniques grouped under the label “post-embedding”, it is obviously necessary to section cells or tissues in some manner before labelling reactions are made. The ideal situation would be, perhaps, use a device such as tissue chopper vibratome, which could unembedded, fixed thin as, say, 100 nm. This currently not possible and doubtful, say least, whether undamaged sections of unsupported ever be Since there no method capable doing this at present, an embedding medium becomes prerequisite. Clearly, stringent conditions attached any that general fine-structure immunocytochemistry.