In Vitro Antibody Formation by Human Tonsil Lymphocytes

摘要: In vitro antibody formation by human lymphocytes was observed against hapten-protein conjugates using palatine tonsil cells in the presence of an optimum concentration 2-mercaptoethanol. Relatively pure populations B and T were prepared method Ficoll-Hypaque gradient centrifugation to investigate cellular mechanism lymphocytes. Maximum response when mixed at optimal ratio. Little occurred each cell alone cultured with antigen. Depletion glass-adherent also resulted diminution anti-hapten response. These data indicated that three kinds cells—T cells, cells—are essentially necessary for primary cells.