Further studies of a spectrophotometric method for the determination of diamine oxidase activity
作者： B. Holmstedt , L. Larsson , R. Tham
DOI: 10.1016/0006-3002(61)90530-3
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摘要: Abstract 1. If diamine oxidase is allowed to act upon the putrescine, γ-amino-butyraldehyde formed. This compound condensed Δ1-pyrroline, which in turn can be reacted with o-aminobenzaldehyde form a yellow compound. A photometric method for determination of has been based on these reactions. 2. Solutions γ-aminobutyraldehyde obtained by hydrolysis from corresponding acetal known concentrations, have react o-aminobenzaldehyde, and resultant extinction measured. In this way calibration curve makes it possible convert units activity micromoles consumed per mg enzyme hour. 3. The manometric spectrophotometric purified agree. tissue homogenates partly enzymes measurements are uninfluenced occurrence other oxidative such as catalase peroxidase.
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