The Arabidopsis class I TCP transcription factor AtTCP11 is a developmental regulator with distinct DNA-binding properties due to the presence of a threonine residue at position 15 of the TCP domain.

摘要: The TCP domain is a DNA-binding present in plant transcription factors that modulate different processes. In the study, we show Arabidopsis class I proteins are able to interact with dyad-symmetric sequence composed of two GTGGG half-sites. TCP20 establishes symmetric interactions 5′ half each strand, whereas TCP11 interacts mainly 3′ half. SELEX (systematic evolution ligands by exponential enrichment) experiments TCP15 and indicated these have similar, although not identical, preferences non-palindromic binding sites type GTGGGNCCNN. shows specificity, preference for GTGGGCCNNN. distinct properties due presence threonine residue at position 15 domain, occupied an arginine most proteins. also forms heterodimers increased efficiency. expression plants repressor form demonstrated this protein developmental regulator influences growth leaves, stems petioles, pollen development. results suggest changes may be one mechanisms through which achieve functional specificity. Abbreviations: AtTCP, thaliana TCP; bHLH, basic helix–loop–helix; CaMV, cauliflower mosaic virus; CYC, cycloidea; EMSA, electrophoretic mobility-shift assay; GUS, β-glucuronidase; MBP, maltose-binding protein; SELEX, systematic enrichment; TB1, teosinte branched 1; UTR, untranslated region; X-gluc, 5-bromo-4-chloro-3-indolyl-β-D-glucuronic acid