Efficient DNA extraction method from soil infested with Fusarium sp. for metagenomics study

摘要: Simple and rapid identification of pathogenic fungi from infested soil is pre-requisite to study the fungal dynamics in soil. The traditional methods characterization are more cumbersome non-specific. Therefore, a specific method through genomic DNA precise. Hence, present study, samples were collected randomly for isolation. concentration ranged between 240 410 ng/μlwith A260/280 values 1.48 1.69. quality was confirmed by restriction digestion with Hind III Hae enzymes further PCR amplification ITS primers. Sequencing NCBI-BLAST analysis of420bp amplicon presence Fusarium spp. sequence submitted NCBI database (Accession no.: KP153547 KP153550). Homology search NCBI-BLAST, found 94–96% homologues Thus, protocol used helps detection borne also gives information about facilitates proper disease management.