Regulation of intracellular pH by electrogenic Na+/HCO3- co-transporters in embryonic neural stem cell-derived radial glia-like cells.

摘要: A stroke causes a hypoxic brain microenvironment that alters neural cell metabolism resulting in membrane hyperpolarization and intracellular acidosis. We studied how pH (pHi) is regulated differentiated mouse progenitor cells during hyperpolarizing conditions, induced by prompt reduction of the extracellular K+ concentration. found radial glia-like population differentiating embryonic cells, but not neuronal was rapidly acidified under these conditions. However, when calcium removed, an instant depolarization recovery pHi, back to normal levels, took place. The rapid phase seen absence calcium, dependent on bicarbonate could be inhibited S0859, potent Na/HCO3 cotransporter inhibitor. Immunostaining PCR data, showed NBCe1 (SLC4A4) NBCn1 (SLC4A7) were expressed pHi glial-like after removal mediated mainly electrogenic sodium transporter (SLC4A4). Our results indicate might hamper regulation activity injury microenvironment. findings show NBC-type transporters are main regulating systems prevailing sensitive important for proliferation, survival stem differentiation.