FLOW CYTOMETRY FOR BIOTECHNOLOGY

摘要: Flow cytometry is a sensitive and quantitative platform for the measurement of particle fluorescence. In flow cytometry, particles in sample single file through focused laser beam at rates hundreds to thousands per second. During time each beam, on order ten microseconds, one or more fluorescent dyes associated with that are excited. The fluorescence emitted from collected microscope objective, spectrally filtered, detected photomultiplier tubes. uniquely capable precise molecular analysis genomic sequence information, interactions between purified biomolecules cellular function. Combined automated handling increased throughput, these features make versatile applications many stages drug discovery. Traditionally, studied cells, especially blood cells; used extensively immunology. This volume shows how integrated into modern biotechnology, dealing issues content, sensitivity, high throughput informatics genomics, proteomics protein-protein interactions, discovery, vaccine development, plant reproductive biology, pharmacology toxicology, cell-cell protein engineering.