Detection of a ryanodine receptor target-site mutation in diamide insecticide resistant fall armyworm, Spodoptera frugiperda.

摘要: Background Fall armyworm (FAW), Spodoptera frugiperda (J.E. Smith), a major lepidopteran pest in Latin and North America, has very recently invaded the continents of Africa Asia. FAW evolved resistance to different insecticides transgenic corn expressing Bacillus thuringiensis (Bt) toxins. Here, we investigated extent mechanisms diamide Brazilian field-collected strain selected using chlorantraniliprole. Results Continuous laboratory selection with chlorantraniliprole resulted ratios 225-fold > 5400-fold against flubendiamide, respectively, when compared susceptible strain. Pre-exposure synergists known inhibit detoxification enzymes did not result significantly increased larval toxicity, suggesting minor role for metabolic resistance. Sequencing ryanodine receptor (RyR) C-terminal domains II VI revealed single nucleotide polymorphism, resulting I4734M mutation said confer target-site diamides pests. Genotyping by pyrosequencing larvae sampled 2018 crop season suggests low allele frequency. Furthermore, developed fluorescent polymerase chain reaction (PCR)-based allelic discrimination assay rapid genotyping samples, because are increasingly used Bt-/non-Bt corn. Conclusions Recently, identified RyR been shown field other pests such as diamondback moth, tomato leafminer striped rice stem borer. The PCR-based will help monitor frequency future spread populations implement appropriate management measures. © 2019 Society Chemical Industry.