A Novel Glyceraldehyde-3-Phosphate Dehydrogenase ( GAPDH ) Promoter for Expressing Transgenes in the Halotolerant Alga Dunaliella salina
作者： Yanlong Jia , Shenke Li , George Allen , Shuying Feng , Lexun Xue
DOI: 10.1007/S00284-012-0102-Y
关键词:
摘要: A major challenge for efficient transgene expression in Dunaliella salina is to find strong endogenous promoters drive the expression. In present study, a novel glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter was cloned and used expressions of bialaphos resistance (bar) gene N-terminal fragment human canstatin (Can-N). The results showed that bar transcribed by GAPDH integrated into genome transformants D. salina. Furthermore, PCR identification, Southern western blots indicated Can-N expressed transgenic salina, demonstrating suitable driving heterologous genes
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sci-hub.se 参考文章(28)
Yu-Min Lü, Xiang-Li Niu, Guo-Zhong Jiang, Le-Xun Xue, The actin gene promoter-driven bar as a dominant selectable marker for nuclear transformation of Dunaliella salina. Acta Genetica Sinica. ,vol. 32, pp. 424- 433 ,(2005)
Song Qin, Congping Tan, Fangqing Zhao, Qun Zhang, Peng Jiang, Establishment of a micro-particle bombardment transformation system for Dunaliella salina. Journal of Microbiology. ,vol. 43, pp. 361- 365 ,(2005)
Masahiro Tamoi, Haruo Kanaboshi, Hitoshi Miyasaka, Shigeru Shigeoka, Molecular mechanisms of the resistance to hydrogen peroxide of enzymes involved in the calvin cycle from halotolerant Chlamydomonas sp. W80. Archives of Biochemistry and Biophysics. ,vol. 390, pp. 176- 185 ,(2001) , 10.1006/ABBI.2001.2375
Chun-Yi Kuo, Shu-Yu Chou, Ching-Tsan Huang, Cloning of glyceraldehyde-3-phosphate dehydrogenase gene and use of the gpd promoter for transformation in Flammulina velutipes. Applied Microbiology and Biotechnology. ,vol. 65, pp. 593- 599 ,(2004) , 10.1007/S00253-004-1635-1
A. Hosseini Tafreshi, M. Shariati, Dunaliella biotechnology: methods and applications Journal of Applied Microbiology. ,vol. 107, pp. 14- 35 ,(2009) , 10.1111/J.1365-2672.2009.04153.X
Jie Li, Lexun Xue, Hongxia Yan, Lili Wang, Lingling Liu, Yumin Lu, Hua Xie, The nitrate reductase gene-switch: a system for regulated expression in transformed cells of Dunaliella salina. Gene. ,vol. 403, pp. 132- 142 ,(2007) , 10.1016/J.GENE.2007.08.001
Jie Li, Lexun Xue, Hongxia Yan, Hongtao Liu, Jianyang Liang, Inducible EGFP expression under the control of the nitrate reductase gene promoter in transgenic Dunaliella salina Journal of Applied Phycology. ,vol. 20, pp. 137- 145 ,(2008) , 10.1007/S10811-007-9197-0
Michael J. Holland, Janice P. Holland, Isolation and identification of yeast messenger ribonucleic acids coding for enolase, glyceraldehyde-3-phosphate dehydrogenase, and phosphoglycerate kinase. Biochemistry. ,vol. 17, pp. 4900- 4907 ,(1978) , 10.1021/BI00616A007
Sabeeha S Merchant, Simon E Prochnik, Olivier Vallon, Elizabeth H Harris, Steven J Karpowicz, George B Witman, Astrid Terry, Asaf Salamov, Lillian K Fritz-Laylin, Laurence Maréchal-Drouard, Wallace F Marshall, Liang-Hu Qu, David R Nelson, Anton A Sanderfoot, Martin H Spalding, Vladimir V Kapitonov, Qinghu Ren, Patrick Ferris, Erika Lindquist, Harris Shapiro, Susan M Lucas, Jane Grimwood, Jeremy Schmutz, Pierre Cardol, Heriberto Cerutti, Guillaume Chanfreau, Chun-Long Chen, Valérie Cognat, Martin T Croft, Rachel Dent, Susan Dutcher, Emilio Fernández, Hideya Fukuzawa, David González-Ballester, Diego González-Halphen, Armin Hallmann, Marc Hanikenne, Michael Hippler, William Inwood, Kamel Jabbari, Ming Kalanon, Richard Kuras, Paul A Lefebvre, Stéphane D Lemaire, Alexey V Lobanov, Martin Lohr, Andrea Manuell, Iris Meier, Laurens Mets, Maria Mittag, Telsa Mittelmeier, James V Moroney, Jeffrey Moseley, Carolyn Napoli, Aurora M Nedelcu, Krishna Niyogi, Sergey V Novoselov, Ian T Paulsen, Greg Pazour, Saul Purton, Jean-Philippe Ral, Diego Mauricio Riaño-Pachón, Wayne Riekhof, Linda Rymarquis, Michael Schroda, David Stern, James Umen, Robert Willows, Nedra Wilson, Sara Lana Zimmer, Jens Allmer, Janneke Balk, Katerina Bisova, Chong-Jian Chen, Marek Elias, Karla Gendler, Charles Hauser, Mary Rose Lamb, Heidi Ledford, Joanne C Long, Jun Minagawa, M Dudley Page, Junmin Pan, Wirulda Pootakham, Sanja Roje, Annkatrin Rose, Eric Stahlberg, Aimee M Terauchi, Pinfen Yang, Steven Ball, Chris Bowler, Carol L Dieckmann, Vadim N Gladyshev, Pamela Green, Richard Jorgensen, Stephen Mayfield, Bernd Mueller-Roeber, Sathish Rajamani, Richard T Sayre, Peter Brokstein, Inna Dubchak, David Goodstein, Leila Hornick, Y Wayne Huang, Jinal Jhaveri, Yigong Luo, Diego Martínez, Wing Chi Abby Ngau, Bobby Otillar, Alexander Poliakov, Aaron Porter, Lukasz Szajkowski, Gregory Werner, Kemin Zhou, Igor V Grigoriev, Daniel S Rokhsar, Arthur R Grossman, None, The Chlamydomonas Genome Reveals the Evolution of Key Animal and Plant Functions Science. ,vol. 318, pp. 245- 250 ,(2007) , 10.1126/SCIENCE.1143609
Federico Valverde, José M. Ortega, Manuel Losada, Aurelio Serrano, Sugar-mediated transcriptional regulation of the Gap gene system and concerted photosystem II functional modulation in the microalga Scenedesmus vacuolatus Planta. ,vol. 221, pp. 937- 952 ,(2005) , 10.1007/S00425-005-1501-0