Concomitant accumulation of intracellular free calcium and arachidonic acid in the ischemic-reperfused rat heart
作者： Tamás Ivanics , Zsuzsa Miklós , László Dézsi , Kornélia Ikrényi , András Tóth
关键词:
摘要: This study was designed to elucidate the relationship between enhanced cytoplasmic calcium levels (Ca2+i) and membrane phospholipid degradation, a key step in loss of cellular integrity during cardiac ischemia/reperfusion-induced damage. Isolated rat hearts were subjected 15 min ischemia followed by 30 reperfusion. Ca2+i estimated Indo-1 fluorescence ratio technique. Degradation phospholipids as indicated increase tissue arachidonic acid content assessed samples taken from myocardium at various points ischemia/reperfusion period. The hemodynamic parameters showed almost complete recovery Fluorescence increased significantly ischemia, but considerable heart-to-heart variation Based upon type change reperfusion, allotted two separate subgroups. Normalization associated with low post-ischemic levels. In contrast, elevated coincided observation is suggestive for Ca2+-related accumulation probably due calcium-mediated stimulation phospholipase A2.
springer.com
sci-hub.se 参考文章(33)
Ashley P. Allshire, Peter H. Cobbold, Causes and effects of changes in cytosolic free calcium in the hypoxic myocardial cell Pathophysiology of Severe Ischemic Myocardial Injury. ,vol. 104, pp. 297- 314 ,(1990) , 10.1007/978-94-009-0475-0_15
S L Hazen, D A Ford, R W Gross, Activation of a membrane-associated phospholipase A2 during rabbit myocardial ischemia which is highly selective for plasmalogen substrate. Journal of Biological Chemistry. ,vol. 266, pp. 5629- 5633 ,(1991) , 10.1016/S0021-9258(19)67641-6
D S Damron, D R Van Wagoner, C S Moravec, M Bond, Arachidonic acid and endothelin potentiate Ca2+ transients in rat cardiac myocytes via inhibition of distinct K+ channels. Journal of Biological Chemistry. ,vol. 268, pp. 27335- 27344 ,(1993) , 10.1016/S0021-9258(19)74254-9
J. C. Miller, R. L. Jones, K. R. Chien, L. M. Buja, H. K. Hagler, J. T. Willerson, Association between inhibition of arachidonic acid release and prevention of calcium loading during ATP depletion in cultured rat cardiac myocytes. American Journal of Pathology. ,vol. 135, pp. 541- 556 ,(1989)
Hans Michael Piper, Irreversible ischemic injury — definition of the problem Pathophysiology of Severe Ischemic Myocardial Injury. ,vol. 104, pp. 3- 14 ,(1990) , 10.1007/978-94-009-0475-0_1
G. J. van der Vusse, J. F. Glatz, H. C. Stam, R. S. Reneman, Fatty acid homeostasis in the normoxic and ischemic heart. Physiological Reviews. ,vol. 72, pp. 881- 940 ,(1992) , 10.1152/PHYSREV.1992.72.4.881
G J van der Vusse, T H Roemen, F W Prinzen, W A Coumans, R S Reneman, Uptake and tissue content of fatty acids in dog myocardium under normoxic and ischemic conditions. Circulation Research. ,vol. 50, pp. 538- 546 ,(1982) , 10.1161/01.RES.50.4.538
L.A. Blatter, W.G. Wier, Intracellular diffusion, binding, and compartmentalization of the fluorescent calcium indicators indo-1 and fura-2 Biophysical Journal. ,vol. 58, pp. 1491- 1499 ,(1990) , 10.1016/S0006-3495(90)82494-2
Franklin H. Epstein, Joseph Y. Cheung, Joseph V. Bonventre, Charles D. Malis, Alexander Leaf, Calcium and ischemic injury The New England Journal of Medicine. ,vol. 314, pp. 1670- 1676 ,(1986) , 10.1056/NEJM198606263142604
H C Lee, R Mohabir, N Smith, M R Franz, W T Clusin, Effect of ischemia on calcium-dependent fluorescence transients in rabbit hearts containing indo 1. Correlation with monophasic action potentials and contraction. Circulation. ,vol. 78, pp. 1047- 1059 ,(1988) , 10.1161/01.CIR.78.4.1047