Electrochemical K-562 cells sensor based on origami paper device for point-of-care testing

摘要: A low-cost, simple, portable and sensitive paper-based electrochemical sensor was established for the detection of K-562 cell in point-of-care testing. The hybrid material 3D Au nanoparticles/graphene (3D NPs/GN) with high specific surface area ionic liquid (IL) widened windows improved good biocompatibility conductivity modified on paper working electrode (PWE) by classic assembly method then employed as sensing surface. IL could not only enhance electron transfer ability but also provide recognition interface conjugation Con cells, capture efficiency sensitivity biosensor strengthened simultaneously. Concanavalin (Con A) immobilization matrix used to cells. As proof-of-concept, cells developed. With such sandwich-type assay format, model were captured A/IL/3D AuNPs@GN/PWE. A-labeled dendritic PdAg NPs Such worked catalysts promoting oxidation thionine (TH) H2O2 which released from via stimulation phorbol 12-myristate-13-acetate (PMA). Therefore, current signal response dependent amount concentration H2O2, latter corresponded releasing So, Under optimized experimental conditions, 1.5×10(-14) mol each calculated. linear range limit determined be 1.0×10(3)-5.0×10(6) cells/mL 200 cells/mL, respectively. as-prepared showed excellent analytical performance fabrication reproducibility, acceptable precision satisfied accuracy, providing a novel protocol testing