Molecular characterization of TMPRSS2-ERG gene fusion in the NCI-H660 prostate cancer cell line: a new perspective for an old model

摘要: Recent studies have established that a significant fraction of prostate cancers harbor signature gene fusion between the 5V region androgen-regulated TMPRSS2 and an ETS family transcription factor, most commonly ERG. Studies on molecular mechanisms functional consequences this important chromosomal rearrangement are currently limited to VCaP cell line derived from vertebral bone metastasis hormone-refractory tumor. Here we report NCI-H660 line, metastatic site extrapulmonary small carcinoma arising prostate. harbors TMPRSS2–ERG with homozygous intronic deletion We demonstrate by real-time quantitative polymerase chain reaction, two-stage dual-color interphase fluorescence in situ hybridization (FISH) assay testing for ERG break-aparts, single-nucleotide polymorphism oligonucleotide arrays. The is consistent common found chromosome 21q22.2–3 human cancer samples. physical juxtaposition DNA level fiber FISH. androgen receptor–negative expresses androgen-independent fashion. This vitro model system has potential provide pathobiologic insights into cancer. Neoplasia (2007) 9, 200 –206