The origins of DNA breaks: a consequence of DNA damage, DNA repair, or apoptosis?

摘要: DNA breaks can arise from many sources after incubation of cells with toxic agents. Very few agents break directly, rather most occur as a result metabolic participation by the cell, such during attempts to repair damage. It is now realized that consequence steps in pathway cell death. Upon reanalyzing methodology commonly used detect breaks, it evident studies would not have observed associated Frequently experimental conditions been are extremely justification were still viable measured their ability exclude dyes trypan blue. However, digestion death apoptosis occurs prior changes membrane integrity. Because possibility endogenous endonuclease activity was realized, may inaccurately assumed arose during, for example, inhibition or intermediates recombination. In light new understanding and formation an early event death, important both reevaluate past conclusions ensure future fully consider derived cytotoxicity every agent under investigation.