Minocycline protects against hepatic ischemia/reperfusion injury in a rat model.

摘要: Hepatic ischemia/reperfusion (I/R) injury is a common clinical problem. The present study was conducted to investigate the protective effect and mechanism of minocycline (Mino), tetracycline with anti-inflammatory antioxidant properties, on I/R liver in rats. In total, 54 male Sprague-Dawley rats were randomly divided into 3 groups 18 each: Sham-operated (control group), model (I/R group) Mino preconditioning (Mino group). group administered (45 mg/kg) by gastric irrigation at 36 h before surgery subsequently 22.5 mg/kg every 12 for surgery. sacrificed 2, 6 24 after reperfusion, serum levels alanine aminotransferase (ALT), aspartate (AST) lactate dehydrogenase (LDH) measured. Hematoxylin/eosin staining tissues performed detect rat histological changes grade (Suzuki's criteria); malondialdehyde (MDA) myeloperoxidase (MPO) determined spectrophotometry; hepatic tumor necrosis factor-α (TNF-α) interleukin-1β (IL-1β) mRNA measured quantitative polymerase chain reaction; Dickkopf-1 (DKK-1) β-catenin gene products detected western blot analysis. treatment significantly ameliorated liver, as shown decreased Suzuki scores function (ALT, AST LDH). After compared MDA MPO TNF-α IL-1β too. protein expression DKK-1 decreased, whereas increased, which indicates that Wnt/β-catenin pathway has been activated. conclusion, protects from mainly through reducing oxidative stress inhibiting release pro-inflammatory cytokines activating signaling liver.