Characterisation of histamine-producing bacteria from farmed blackspot seabream (Pagellus bogaraveo) and turbot (Psetta maxima).

摘要: Abstract Turbot ( Psetta maxima ) and blackspot seabream Pagellus bogaraveo represent two of the most important emerging farmed fish species in European countries. However, no information presence development histamine-producing bacteria on them has been reported so far. Accordingly, aim this study was to isolate identify main turbot seabream. For study, 24 isolates (12 from 12 seabream) were preliminarily selected Niven medium. Two these confirmed as prolific histamine producers by HPLC. Thus, Pseudomonas fragi (isolated turbot) syringae able produce 272 ± 69 ppm 173 ± 45 ppm vitro, respectively, after incubation at 30 °C/24 h. While fillets proved be quite resistant formation temperatures below 10 °C, inoculated with P. former Morganella morganii accumulated 696 ± 84 760 ± 59 ppm histamine, under such conditions. Genetic identification based 16S rRNA sequencing performed parallel investigation characteristic mass spectral profiles matrix-assisted laser desorption/ionisation time-of-flight spectrometry (MALDI-TOF MS). The MALDI-TOF MS analyses provided species-specific fingerprints, which allow rapid classification isolates. Six genus-specific peaks range 2218–4434 m/z shared both strains. Bacterial achieved six ranges 2534–7183 2536–9113 for , respectively.