Accessory cell-derived IL-12 and prostaglandin E2 determine the IFN-gamma level of activated human CD4+ T cells.

摘要: IL-12 and PGE2 are two immunomodulators produced by accessory cells (AC) in response to various stimuli. IL- 12 enhances IFN-gamma production activated CD4+ T cells, whereas inhibits the secretion of this cytokine. Because these AC-derived factors exert clearly opposite modulatory effects on production, we examined 1) net-IFN-gamma stimulated presence both PGE2, 2) susceptibility time adding modulators at different timepoints after stimulation, 3) relative contributions levels stimulating LPS-activated monocytes inhibitors or IL-12. Here, demonstrate that do not abrogate action each other is determined their concentration ratio, become insensitive retained activation, potently modulate via release PGE2. The shift time, due kinetics, from a dominant effect mixed IL-12/PGE2 effect. net largely ratio timepoint cell an imbalance may, therefore, lead immunologic dysfunction.