Exploring the diversity and hydrocarbon bioremediation potential of microbial community in the waste sludge of Duliajan oil field, Assam, India.

摘要: Microbial community analysis of crude oil containing sludge collected from Duliajan field, Assam, India, showed the predominance hydrocarbon-degrading bacteria such as Pseudomonas (20.1%), Pseudoxanthomonas (15.8%), Brevundimonas (1.6%), and Bacillus (0.8%) alongwith anaerobic, fermentative, nitrogen-fixing, nitrate-, sulfate-, metal-reducing, syntrophic bacteria, methanogenic archaea. Phylogenetic Investigation Communities by Reconstruction Unobserved States (PICRUSt) indicated gene collection for potential hydrocarbon degradation, lipid, nitrogen, sulfur, methane metabolism. The culturable microbial was predominated with metabolic utilizing diverse hydrocarbons, oil, actual petroleum sole carbon source during growth tolerating various environmental stresses prevailing in contaminated sites. More than 90% isolated strains could produce biosurfactant exhibit catechol 2,3-dioxygenase activity. Nearly 30% isolates alkane hydroxylase activity maximum specific 0.54 μmol min-1 mg-1. study provided better insights into diversity functional within which be exploited situ bioremediation