Incomplete reactivation of Oct4-related genes in mouse embryos cloned from somatic nuclei.

摘要: The majority of cloned animals derived by nuclear transfer from somatic cell nuclei develop to the blastocyst stage but die after implantation. Mouse embryos that lack an Oct4 gene, which plays essential role in control developmental pluripotency, and also implantation, because they pluripotent embryonic cells. Based on this similarity, we posited differentiated fail establish a population truly cells faulty reactivation key genes such as Oct4. To explore hypothesis, used silico approach identify set Oct4-related whose expression pattern is similar When 10 was analyzed individual cumulus cell-derived blastocysts, only 62% correctly expressed all tested genes. In contrast incomplete clones, ES blastocysts normal these normally. Notably, between patterns correlated with efficiency development term. These observations suggest failure reactivate full spectrum may contribute lethality somatic-cell clones.