A modular approach to the design of protein-based smart gels
作者： Tijana Z. Grove , Jason Forster , Genaro Pimienta , Eric Dufresne , Lynne Regan
DOI: 10.1002/BIP.22033
关键词:
摘要: The modular nature of repeat proteins makes them a versatile platform for the design smart materials with predetermined properties. Here, we present general strategy combining protein modules specified stability and function into arrays assembly stimuli-responsive gels. We have designed tetratricopeptide (TPR) which contain peptide-binding that specify strength reversibility network crosslinking in combination spacer geometry overall array. By such multivalent peptide ligands, self-supporting gels are formed. Using microrheology, characterized kinetics gelation as concentration stoichiometry components. also show effective encapsulating releasing small molecules. Moreover, TPR alone fully compatible cell growth, whereas loaded an anticancer compound release compound, resulting death. Thus, demonstrated this new class tunable biomaterials is ripe further development tissue engineering drug delivery platform.
sci-hub.se 参考文章(37)
Clemens Scheufler, Achim Brinker, Gleb Bourenkov, Stefano Pegoraro, Luis Moroder, Hans Bartunik, F.Ulrich Hartl, Ismail Moarefi, Structure of TPR Domain–Peptide Complexes: Critical Elements in the Assembly of the Hsp70–Hsp90 Multichaperone Machine Cell. ,vol. 101, pp. 199- 210 ,(2000) , 10.1016/S0092-8674(00)80830-2
Tommi Kajander, Aitziber L. Cortajarena, Ewan R. G. Main, Simon G. J. Mochrie, Lynne Regan, A new folding paradigm for repeat proteins Journal of the American Chemical Society. ,vol. 127, pp. 10188- 10190 ,(2005) , 10.1021/JA0524494
Nicole H. Romano, Debanti Sengupta, Cindy Chung, Sarah C. Heilshorn, Protein-engineered biomaterials: Nanoscale mimics of the extracellular matrix Biochimica et Biophysica Acta (BBA) - General Subjects. ,vol. 1810, pp. 339- 349 ,(2011) , 10.1016/J.BBAGEN.2010.07.005
Tommi Kajander, Aitziber L. Cortajarena, Simon Mochrie, Lynne Regan, Structure and stability of designed TPR protein superhelices: unusual crystal packing and implications for natural TPR proteins. Acta Crystallographica Section D-biological Crystallography. ,vol. 63, pp. 800- 811 ,(2007) , 10.1107/S0907444907024353
Ewan RG Main, Sophie E Jackson, Lynne Regan, The folding and design of repeat proteins: reaching a consensus Current Opinion in Structural Biology. ,vol. 13, pp. 482- 489 ,(2003) , 10.1016/S0959-440X(03)00105-2
Monica C. Branco, Joel P. Schneider, Self-assembling materials for therapeutic delivery Acta Biomaterialia. ,vol. 5, pp. 817- 831 ,(2009) , 10.1016/J.ACTBIO.2008.09.018
Jennifer S. Haghpanah, Carlo Yuvienco, Eric W. Roth, Alice Liang, Raymond S. Tu, Jin Kim Montclare, Supramolecular assembly and small molecule recognition by genetically engineered protein block polymers composed of two SADs. Molecular BioSystems. ,vol. 6, pp. 1662- 1667 ,(2010) , 10.1039/C002353A
Meredith E. Jackrel, Roberto Valverde, Lynne Regan, Redesign of a protein-peptide interaction: characterization and applications. Protein Science. ,vol. 18, pp. 762- 774 ,(2009) , 10.1002/PRO.75
Ronak V. Rughani, Daphne A. Salick, Matthew S. Lamm, Tuna Yucel, Darrin J. Pochan, Joel P. Schneider, Folding, Self-Assembly, and Bulk Material Properties of a De Novo Designed Three-Stranded β-Sheet Hydrogel Biomacromolecules. ,vol. 10, pp. 1295- 1304 ,(2009) , 10.1021/BM900113Z
Nicolynn E. Davis, Sheng Ding, Ryan E. Forster, Daniel M. Pinkas, Annelise E. Barron, Modular enzymatically crosslinked protein polymer hydrogels for in situ gelation. Biomaterials. ,vol. 31, pp. 7288- 7297 ,(2010) , 10.1016/J.BIOMATERIALS.2010.06.003