The contribution of electron microscopy to the differential diagnosis of tumors.

摘要: Summary From these selected examples of our own experience, as well from the results obtained by others (Rosai and Rodriguez, 1968), it must be concluded that electron microscopy can, on occasion, a significant aid in accurate diagnosis various neoplasms. While optimal fixation tissues processed for produces excellent with good cytologic preservation cellular organelles extracellular components, use should not excluded because tissue has been fixed formalin or routinely embedded paraffin light microscopy. Today, rapid minced glutaraldehyde postfixation osmium tetroxide provides best fine structure cells (Sabatini et al., 1963). However, can if, instead glutaraldehyde, minute pieces are originally buffered formaldehyde according to standard microscopic techniques. Furthermore, larger fragments stored prolonged periods time still useful diagnostic since many ultrastructural features used purposes preserved even fixation. Carson al. (1973) have shown phosphate commercial satisfactory routine In past investigators suggested fixatives substitutes usual which could enhance (Chambers 1968). all either financially unfeasible large scale provided suboptimal specimens. Recently McDowell Trump (1975) introduced mixture 4% 1% fixative which, investigators, gives automated histologic processing studies. But, also re-emphasized is possible only shortly thereafter, but when had time, paraffin. A method reprocessing formalin-fixed wet tissue, described (Zimmerman 1972), sections paraffin-embedded postmortem specimens prepared study what called “open-face” embedding (Rossi 1970).