Avian influenza virus (H5N1); effects of physico-chemical factors on its survival

摘要: Present study was performed to determine the effects of physical and chemical agents on infective potential highly pathogenic avian influenza (HPAI) H5N1 (local strain) virus recently isolated in Pakistan during 2006 outbreak. having titer 108.3 ELD50/ml mixed with sterilized peptone water get final dilution 4HA units then exposed (temperature, pH ultraviolet light) (formalin, phenol crystals, iodine CID 20, virkon®-S, zeptin 10%, KEPCIDE 300, 400, lifebuoy, surf excel caustic soda) agents. Harvested amnio-allantoic fluid (AAF) from embryonated chicken eggs inoculated treated (0.2 ml/egg) subjected haemagglutination (HA) inhibition (HI) tests. lost infectivity after 30 min at 56°C, 1 day 28°C but remained viable for more than 100 days 4°C. Acidic (1, 3) basic (11, 13) were virucidal 6 h contact time; however retained 5 (18 h), 7 9 (more 24 h). UV light proved ineffectual inactivating completely even 60 min. Soap (lifebuoy®), detergent (surf excel®) alkali (caustic destroyed 0.1, 0.2 0.3% dilution. All commercially available disinfectants inactivated recommended concentrations. Results present would be helpful implementing bio-security measures farms/hatcheries levels wake (AIV)