Use of two-dimensional 1H nuclear magnetic resonance to study high-affinity antibody-peptide interactions

摘要: Abstract In general, we can conclude that, while in some cases changes the coupling constants within a given residue may contribute to observed intensities of COSY cross-peaks bound peptide spectrum, for many their absence from spectrum will also reflect an increase linewidth corresponding resonances which peak derives. Moreover, since peptides undergo rapid conformational averaging solution,53,54 J values are likely correspond localization amino acid side chains specific antibody: interactions. From analysis peptide, as described above, therefore extract information pertaining both dynamic behavior residues when antibody combining site, and degree interaction individual with those complex.