Establishment and characterization of the NCC-SS1-C1 synovial sarcoma cell line

摘要: Synovial sarcoma is an aggressive mesenchymal malignancy characterized by unique gene fusions. Tissue culture cells are essential tools for further understanding tumorigenesis and anti-cancer drug development; however, only a limited number of well-characterized synovial cell lines exist. Thus, the objective this study was to establish patient-derived line. We established line from tumor tissue isolated 72-year-old female patient. Prepared were analyzed presence fusions fluorescence in situ hybridization, RT-PCR, karyotyping. In addition, resulting viability, short tandem repeat, colony spheroid formation, invasion analyses. Differences enrichment between primary examined mass spectrometric protein expression profiling KEGG pathway analysis. Our analyses revealed that NCC–SS1–C1 harbored SS18–SSX1 fusion typical similar proteomics profiles. vitro also confirmed invasive, colony-forming, spheroid-forming potentials. Moreover, screening with chemotherapeutic agents tyrosine kinase inhibitors doxorubicin, subset inhibitors, several molecular targeting drugs markedly decreased viability. Results present support will be effective tool research.