Cytological and iTRAQ-based quantitative proteomic analyses of hau CMS in Brassica napus L.

摘要: Hau cytoplasmic male sterility (CMS) is a new type of CMS that was originally identified in Brassica juncea and subsequently transferred to B. napus rapa. To further elucidate the molecular mechanism underlying hau napus, semithin section analysis iTRAQ-based differential proteomic were performed compare its maintainer line. Cytological revealed abnormal anther development line arrested during differentiation stamen archesporial cells. qRT-PCR showed gene orf288 expressed at substantially higher levels anthers than with restored fertility. In comparison line, total 186 differentially abundant proteins 58 which exhibited increased accumulation 128 decreased accumulation. Bioinformatics involved carbohydrate energy metabolism, such as those oxidative phosphorylation, glycolysis/gluconeogenesis pyruvate whereas stress, antagonism cell death protein processing indicating potential roles metabolism supply regulation abortion. BIOLOGICAL SIGNIFICANCE: Cytoplasmic one most efficient ways produce hybrid seeds crops. mainly caused by mitochondrial mutation has been an important model for investigation nuclear interactions various plant species. completely abortive anthers. Although studies have conducted identify key genes associated CMS, mechanisms remain unclear. this study, cytological, molecular, approaches used reveal napus. Based on expression profiles regulatory network proposed herein. These results may provide insights into basis