Localized reconstruction in Scipion expedites the analysis of symmetry mismatches in cryo-EM data

摘要: Abstract Technological advances in transmission electron microscopes and detectors have turned cryogenic microscopy (cryo-EM) into an essential tool for structural biology. A commonly used cryo-EM data analysis method, single particle analysis, averages hundreds of thousands low-dose images individual macromolecular complexes to determine a density map the complex. The presence symmetry complex is beneficial since each projection image can be assigned multiple views However, processing that applies average out asymmetric features consequently methods are required resolve features. Scipion framework integrates functions from different packages as plugins. To extend its functionality handling mismatches, we present here plugin termed LocalRec implementing localized reconstruction method. When tested on adenovirus set, enables resolving symmetry-mismatched trimeric fibre bound five-fold vertices capsid. Furthermore, it improves structure determination icosahedral capsid by dealing with defocus gradient across particle. expected widely applicable range investigations flexible mismatched complexes.