Genes induced by growth arrest in a pancreatic β cell line: identification by analysis of cDNA arrays

摘要: Pancreatic β cell lines are a potentially attractive source of material for therapy insulin-dependent diabetes mellitus. However, induction proliferation in post-mitotic, differentiated cells is likely to affect the expression multiple genes associated with function, resulting dedifferentiation. We have developed murine line by conditional transformation SV40 T antigen oncoprotein. These can undergo reversible and growth arrest. Here we utilized this model identify differences gene between proliferating quiescent cells, analyzing known differentially secreted proteins, as well systematic survey mouse cDNA array. Our findings demonstrate that arrest stimulates insulin encoding components secretory vesicles. Screening array revealed activation following arrest, many them novel which may be related function. Characterization these contribute our understanding function ability employ diabetes.