Influence of oxygen on the proliferation and metabolism of adipose derived adult stem cells.

摘要: Articular cartilage is an avascular connective tissue that exhibits little intrinsic capacity for repair. exists in a reduced oxygen (∼5%) environment vivo; therefore, tension may be important factor regulates the metabolism of chondrocyte progenitors. A number recent studies have developed engineering approaches promoting repair using undifferentiated progenitor cells seeded on biomaterial scaffolds, but known about how might influence these engineered tissues. Human adipose-derived adult stem (hADAS) isolated from stroma subcutaneous fat were suspended alginate beads and cultured control or chondrogenic media either low (5%) atmospheric (20%) up to 14 days. Under conditions, significantly inhibited proliferation hADAS cells, induced two-fold increase rate protein synthesis three-fold total collagen synthesis. Low also increased glycosaminoglycan at certain timepoints. Immunohistochemical analysis showed significant production cartilage-associated matrix molecules, including type II chondroitin-4-sulfate. These findings suggest play role regulating as they undergo chondrogenesis, exogenous provide means increasing overall accumulation macromolecules tissue-engineered cartilage. © 2005 Wiley-Liss, Inc.